Affiliation: Masaryk University, Faculty of Medicine
Study program: PhD program Biomedical Sciences
Specialization: Biochemistry and Molecular Biology
Workplace: Cellular and Molecular Immunoregulation group, Center of Translational
Medicine, ICRC-FNUSA
Mode: Full-time

Supervisor: Mgr. Jan Frič, Ph.D
Consultant: Mgr. Marcela Hortová Kohoutková, Ph.D.

Title: Role of soluble cytokine receptors in control of inflammation

Brief annotation (max 200 words):
Sepsis and septic shock severely remodel the immune system, leaving the patients vulnerable to
recurrent infection and overall decline of health related quality of life. Decline of immune functions,
which is central for many sepsis long-term consequences, can persist many months and years after
sepsis resolution.
The project aims to reveal sepsis-induced remodelling of bone marrow niche and its effect on the
hematopoetic stem and progenitor cells (HSPC) and their innate cells progeny. Specifically, the project
will address the impact of inflammatory cytokines stimulation and regulation role of soluble cytokine
receptors and how these molecules alter hematopoiesis and differentiation of innate immune cells.
This translational project will combine the use of iPSCs derived hematopoiesis, model of bone marrow
niche and analysis of the sepsis dynamics in patients using deep flow cytometry immunophenotyping
combined with analysis of humoral markers of inflammation and tissue damage. Project aims to
dissect the molecular mechanism behind the development of sepsis related immunoparalysis
responsible for increased susceptibility to infections as well as the development of long-term
consequences of sepsis.

Funding: Horizon Europe – BEATsep

Requirements: We seek highly motivated candidate keen to develop translational research of
immunology of acute and chronic inflammation. The candidate will learn large spectra of methodology
including processing of blood samples from the patients and controls, flow cytometry phenotypization,
detection of proteins in plasma samples, RNA expression, advanced cell culture protocols containing
establishing 3D bone marrow model, analysis of functional and molecular markers pathogen