Přehled

Doctoral study program: Life Sciences (Faculty of Science, Masaryk University)

Department: CEITEC MU

Form of study: doctoral full time

Supervisor: RNDr. Radek Marek, Ph.D.

Annotation

The project is focused on detailed structural characterization of short oligonucleotides in noncanonical forms clipped together by proper sequential motifs. For this purpose, NMR experiments combined with MD simulations will be employed. The effect of modifications of selected nucleotides on the structural properties of designed models will be investigated to gain a deeper understanding of key interactions that contribute to the system folding and stability.

Recommended literature

• Aleš Novotný, Jan Novotný, Iva Kejnovská, Michaela Vorlíčková, Radovan Fiala, Radek Marek. Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip. Nucleic Acids Research, 2021, 49, 11425. doi:10.1093/nar/gkab915.

Research area: Structural Biology

Keywords: NMR spectroscopy, noncanonical DNA, base pairing, molecular modelling

Funding of the PhD candidate:

RG budget, planned Czech Science Foundation

Requirements for candidate:

Structural chemistry or biology, advanced NMR spectroscopy, computational chemistry

Information about the supervisor

https://www.ceitec.eu/prof-radek-marek-ph-d/u17384

Currently 2 PhD students, 12 alumni

More information on the Radek Marek Research Group: https://www.ceitec.eu/structure-of-biosystems-and-molecular-materials/rg108

Information about the application process: https://www.ceitec.eu/ls-mm-phd/

Application webpage: https://www.ceitec.eu/junctions-in-noncanonical-forms-of-nucleic-acids/t11586