Tutor Prof. Ing. Michal Hocek, PhD. DSc.
Team Michal Hocek
Topic Modifications of nucleotides and RNA by reactive groups for bioconjugations and cross-links
Abstract Ribonucleotides and RNA bearing reactive functional groups will be designed and synthesized. The modified RNAs will be prepared by polymerase incorporations of modified nucleoside triphosphates or by click reactions on RNA. Bioorthogonal reactions of these groups in RNA will be studied, e.g. cross-linking with proteins, attachment of other useful functional groups and applications in chemical biology and diagnostics.

Leading references:
1. Dadová, J.; Orság, P.; Pohl, R.; Brázdová, M.; Fojta, M.; Hocek, M. „Vinylsulfonamide and acrylamide modification of DNA for cross-linking with proteins“ Angew. Chem. Int. Ed. 2013, 52, 10515-10518.
2. Milisavljevič, N.; Perlíková, P.; Pohl, R.; Hocek, M.: „Enzymatic synthesis of base-modified RNA by T7 RNA polymerase. A systematic study and comparison of 5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside triphosphates as substrates“ Org. Biomol. Chem. 2018, 16, 5800-5807

More information & How to apply
Find more projects and see how to apply at www.iocb.cz/phd. (Please note: Deadline for applications is February 21, 2019; IOCB Interview Day is on March 14, 2019)