Přehled

Group:

Hana Cahová Group

Tutor:

Ing. Hana Macickova Cahova, Ph.D.

Field of study:

Cell Biology

Abstract:

The recent discovery of RNA caps, such as NAD and CoA, has led to a reassessment of RNA structure in all types of cells. In our search for new RNA modifications, we have discovered a brand-new class of 5‘ caps – dinucleoside polyphosphates (NpnN) in bacteria as well as in eukaryotes. The project will focus on the development of a general LC-MS sequencing method employing various selective RNases. The student will use the technique to study the properties of the 5’ end of various non-coding RNAs (such as snRNA and snoRNA), as well as the capping status of natural mRNA (distinguishing between cap1 and cap2). Moreover, the method will be useful for the assessment and quality control of various mRNA vaccines.

References:

Potužník, J.F.; Nešuta, O. et al. Diadenosine Tetraphosphate (Ap4A) Serves as a 5′ RNA Cap in Mammalian Cells, Angewandte Chemie, https://doi.org/10.1002/anie.202314951 (2023)
Hudeček, O., et al. Dinucleoside polyphosphates act as 5’-RNA caps in bacteria Nature Communications, 11, 1052 (2020).
B. Benoni, R. Benoni, J. Trylcova, K. Grab, J. Pačes, J. Weber, D. Stanek, J. Kowalska, L. Bednarova, Z. Keckesova, L. Gahurova, H. Cahova, bioRxiv 2022, 2022.2011.2010.515957.

University:

Faculty of Science, Charles University

How to apply:

At the Institute of Organic Chemistry and Biochemistry of the CAS / IOCB Prague, we are looking for talented, independent, and highly motivated PhD students with an MSc. degree or equivalent in life sciences or related fields. We are offering more than 20 new exciting projects in organic and medicinal chemistry, biochemistry, molecular biology, analytical, and physical chemistry.

  • Online registration deadline: 3 March 2024
  • Interview Day: 21 March 2024

Learn more and apply at www.uochb.cz/en/call-for-phd-applications.