The Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic, is opening a position in the Laboratory of Cellular Neurophysiology in the group of Prof. Ladislav Vyklicky M.D., Ph.D., D.Sc. for a POSTDOCTORAL RESEARCHER to study NMDA receptors.

The postdoctoral position is available for 3 years with a possible extension as a junior scientist.

The main research interest of the laboratory is the structure, function, and pharmacology of NMDA receptors (NMDARs) and their role in excitatory synaptic transmission. The goal is to understand how mutations in genes encoding NMDAR subunits described in individuals diagnosed with neuropsychiatric disorders such as schizophrenia, autism spectrum disorder or epilepsy, affect excitatory signaling and to rectify the impaired function by pharmacological tools. In our research we use mainly electrophysiological (patch-clamp), molecular biology, immunohistochemical, and behavioral methods with the employment of different models. More information about the laboratory profile is available at: https://www.fgu.cas.cz/en/departments/laboratory-of-cellular-neurophysiology.

Annotation of the research project

The research project will focus on the use of single-molecule Förster resonance energy transfer (smFRET) to study conformational dynamics of individual NMDAR molecules with the aim to bridge the gap between electrophysiological and structural studies.  Since smFRET allows conformational readout of transitions between non-conductive states, it provides an ideal method for studying the effect of NMDAR mutations on receptor conformational dynamics and allows identification of exact conformational states in which NMDAR modulators bind and how they affect this conformational space, dynamics, and eventually the NMDAR function.

The successful candidate should have a background in biophysics, neuroscience and pharmacology supported by a strong publication record. He/she will be responsible for independent design of experiments, data acquisition and data analysis. Previous experience in cell cultures, cloning, patch-clamp electrophysiology and smFRET is expected. Basics of Unix and/or Python is a plus.

We offer a competitive monthly salary of 50 000 CZK (approx. 1 800 EUR) with a possible raise based on the candidate’s skills and involvement in grant projects. For further information please contact Marcela Tomášková (Marcela.Tomaskova@fgu.cas.cz). To apply, please send a letter of interest, a structured CV, a list of publications, photocopies of documents proving the attained level of education, and recommendation letters of two independent academic referees to the following address: marcela.tomaskova@fgu.cas.cz    Applications need to be received by  November 30th 2020. 

Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 142 20 – Prague 4, Czech Republic