Přehled

Supervisor: Lukáš Žídek

Annotation: RNA polymerase of B. subtilis contains subunit delta with a large intrinsically disordered region. The effect of the disordered region on biophysical properties of the delta subunit and on transcription rate and cell viability have been described recently. Now we plan to study the mechanistic role of the disordered region in the process of transcription, especially during transcription initiation. Cryo-electron microscopy and NMR will be used to describe structured portion of RNA polymerase and the disordered region of its delta subunit, respectively, to reveal whether the disordered region remains flexible in complex. Based on the results, cryo-EM (focused 3D classification) and NMR (enhanced by selective isotope and paramagnetic labelling, combined with EPR) will be used as alternative and/or complementary approaches. The supervisor will provide the expertise in the field of NMR. Cryo-EM and EPR expertise will be provided by CF and external collaborators. The result should lead to (shared) first-author publication of the student in a respected scientific journal.

Requirements on candidates:

Solid background in physical chemistry or biophysics, knowledge of biochemistry and molecular biology. Experience with NMR spectroscopy or cryo-electron microscopy is an advantage.

Recommended literature:

  • Kuban, V. et al. J. Am. Chem. Soc., 141 (2019) 16817­16828.
  • Pei, H.-H. et al. Nat. Commun., 11 (2020) 6418.
  • Rabatinova, A. et al. J. Bacteriol., 195 (2013) 2603­2611.

More information on CEITEC PhD School website: https://ls-phd.ceitec.cz/ and Research Group website: https://www.ceitec.eu/protein-structure-and-dynamics/rg110

In case you are interested. ​Please, submit your CV and motivation letter via Registration Form.

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