Immune system has evolved to protect the body from invading pathogens and cancer. Yet aberrant inflammation can lead to severe autoimmune diseases. In order to properly regulate activation and termination of the immune responses, individual cells must communicate with each other and provide the information about ongoing inflammation.
One means of communication is the production of small soluble proteins termed cytokines, that are detected by specific receptors on target cells. Proper sensing of these cytokines allow immune cells to activate adequate immune reaction. On the other hand, erroneous response to the cytokine stimulation can lead to severe autoimmunity. The present research aims to uncover new molecular mechanisms how crucial pro-inflammatory cytokines signal and identify new possible venues to modulate their activity in the case of autoimmunity.
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Systematic analysis of the IL-17 receptor signalosome reveals a robust regulatory feedback loop. EMBO J. 2020 Sep 1;39(17):e104202. doi: 10.15252/embj.2019104202. Epub 2020 Jul 21.
TBK1 and IKKε prevent TNF-induced cell death by RIPK1 phosphorylation. Nat Cell Biol. 2018 Dec;20(12):1389-1399. doi: 10.1038/s41556-018-0229-6.
Candidate should be deeply interested in science, proficient in English, willing to collaborate and share experience with colleagues, and willing to work with murine models. The candidate should hold a master degree in immunology, cell biology, biochemistry, molecular biology, or a related field. Prior laboratory experience is advantageous.
The project will involve study of protein complexes via mass-spectrometry, preparation of knockout cell lines via CRISPR/Cas9 approach, and analysis of their signaling using methods of molecular biology. Data obtained from cell lines will be further studied in vivo using mouse models and murine knockout strains. The research project will be carried at BIOCEV research campus in Vestec. We offer monthly salary 20.000 CZK which is supplemented with stipend 15.000 CZK and five weeks of annual paid leave.