Přehled

Ph.D. Project 

DNA damage repair of DNA-protein crosslinks in Arabidopsis thaliana 

Supervisor: Assoc. Prof. Ales Pecinka, Ph.D.  / pecinka@ueb.cas.cz

Cellular processes and external factors generate stress that can damage nuclear DNA. Proteins covalently bound to DNA represent the little-studied but serious type of DNA damage – DNA-protein crosslinks (DPCs). DPCs block transcription and DNA replication and therefore need to be repaired. We have developed a highly efficient genetic screen for the identification of genes involved in DPC repair.

The project:

  • Identification of genes responsible for the repair of DPCs via forward directed genetic screen and mapping-by-sequencing
  • Genetic analysis of the candidates (crossing, complementation, homologous recombination assays)
  • Molecular analyses (work with DNA and RNA, sequencing, PCR, reverse transcription, qPCR, molecular cloning, work with E.coli etc.)

Aims:

  • Reconstructing molecular pathways protecting the plant genome against DPCs

Motivation: 

  • Be part of the motivated team of the Centre of Plant Structural and Functional Genomics of the Institute of Experimental Botany located in the historical university city of Olomouc, the Czech Republic
  • Enjoy extraordinary work conditions and performance based benefits

Contact:

pecinka@ueb.cas.cz