Supervisor:  Mgr. Markéta Pernisová, Ph.D.


The main task of the project will be implementing and optimizing CRISPR/Cas methodology in the laboratory. Set of suitable vectors will be designed by cloning methodology (Gibson assembly, Gateway etc.). Plant mutant lines will be generated firstly in Arabidopsis then in crops like Brassica, wheat, or barley. Genes of interest will be chosen mainly from cytokinin biosynthesis or signalling families, e.g. log genes. Regeneration and cultivation protocols will be established to obtain CRISPR/Cas lines. These protocols are expected to become part of the regular offer of services that are available from the Plant Sciences Core Facility. Results will be published in impacted journals.

Recommended literature:

  • Capdeville N, Merker L, Schindele P, Puchta H.: Sophisticated CRISPR/Cas tools for fine-tuning plant performance. J Plant Physiol. 2021 Feb;257:153332. doi: 10.1016/j.jplph.2020.153332.
  • Chen K, Wang Y, Zhang R, Zhang H, Gao C.: CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. Annu Rev Plant Biol. 2019 Apr 29;70:667-697. doi: 10.1146/annurev-arplant-050718-100049.
  • Knott GJ, Doudna JA.: CRISPR-Cas guides the future of genetic engineering. Science. 2018 Aug 31;361(6405):866-869. doi: 10.1126/science.aat5011.
  • Kumlehn J, Pietralla J, Hensel G, Pacher M, Puchta H.: The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology. J Integr Plant Biol. 2018 Dec;60(12):1127-1153. doi: 10.1111/jipb.12734.

For more detail about our group, please refer to our website: https://www.ceitec.cz/laborator-rostlinneho-vyzkumu/cf123. 

Applications should include: a CV, a scanned copy of the Masters or an official letter certifying its completion, brief motivation letter, and contacts for at least two references should be sent via e-mail marketa.pernisova@ceitec.muni.cz or phd@ceitec.muni.cz.

More information about CEITEC PhD School are available at http://ls-phd.ceitec.cz/.