Overview

Recent discoveries of non-canonical RNA caps, such as NAD and CoA, have led to a fundamental reassessment of the structural diversity of RNA across all domains of life. In our research group, we have identified an entirely new class of RNA caps—dinucleoside polyphosphates (NpnN)—present in both bacterial and eukaryotic cells. This discovery raises new questions regarding the diversity, regulation, and biological function of RNA 5′ ends.
The aim of this project is to develop a robust and reliable RNA sequencing approach based on liquid chromatography–mass spectrometry (LC–MS) combined with the use of selective RNases. The developed methodology will be applied to investigate the properties of 5′ ends of various non-coding RNAs, including snRNAs and snoRNAs, as well as to quantify the methylation status of the canonical mRNA cap, enabling discrimination between cap 1 and cap 2 structures.
Beyond fundamental research, this method will provide a versatile tool for the analysis and quality control of mRNAs used in biotechnological and therapeutic applications, thereby bridging basic RNA biology with applied research.

University:

Faculty of Science, Charles University

Group:

Hana Cahová Group, Chemical Biology of Nucleic Acids

Tutor:

Ing. Hana Macicková Cahová, Ph.D.

Field of study:

Cell biology