Přehled

Sepsis and septic shock frequently severely remodel the immune
system its functionality leading to increased susceptibility to infections. Dynamic changes in immune
phenotype induced by septic insult generate important candidates of new biomarkers predicting
severeness of the syndrome and eventually predicting the survivorship. Moreover, excessive
inflammation leads to development of immunoparalysis, which can persist many months after sepsis
resolution. The project aims to identify patients in the risk of severe immunoparalysis and other
understudied adverse effects. Proposed analysis of the sepsis dynamics using deep flow cytometry
immunophenotyping combined with analysis of humoral markers of inflammation and tissue damage
or various metabolites. This will be complemented by experimental research using different tissue
models, including organoids where the PhD candidate will search for molecular basis of long-term
adverse effect of inflammatory insult. This will include work with mucosal organoids, blood cells from
healthy donors and analysis of various signalling pathways.
Funding: NPO_EXCELLES
Requirements: We seek for motivated candidate keen to develop translational research of immunology
of acute and chronic inflammation. The candidate will learn large spectra of methodology including
processing of blood samples from the patients and controls, flow cytometry phenotypization, detection
of proteins in plasma samples, RNA expression, advanced cell culture protocols, analysis of functional
and molecular markers pathogen recognition.